Kinesin Protocols (Methods in Molecular Biology Vol 164) [1sted.]9780896037663, 9781592590698, 0896037665

Author : isabelle Vernos
Description:Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. Among the approaches considered are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. Comprehensive and highly practical, Kinesin Protocols makes available all the key basic and cutting-edge methods needed to successfully study the multifaceted world of kinesin-like proteins and to explore their many functions.
Categories: Biology Molecular
Year :2001
Publisher :
Language : English
N° Of Pages : 258[271]
File Info : pdf 2 Mb