Methods in Molecular Biology 064Protein Sequencing Protocols [1ed.]9780896033535, 0896033538

Description:A timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on, masters of the procedures. Conveys valuable lessons and insights into using automated sequencers and mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acids by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. Also deals with such troublesome problems as identifying sites of modification and working with very small samples.