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Methods in Molecular Biology 1156Shotgun Proteomics: Methods and Protocols [1ed.]1493906844, 9781493906840

Author : Daniel Martins-de-Souza (eds.)
Description:Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified and characterized in a high throughput manner. Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species.
Categories: Biology Molecular
Year :2014
Publisher : Humana Press
Language : English
N° Of Pages : 492[507]
File Info : pdf 9 Mb

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