Methods in Molecular Biology 1344TGF- Signaling: Methods and Protocols1493929658, 9781493929658

Author : Xin-Hua Feng, Pinglong Xu, Xia Lin
Description:This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF- from the basic principles to the practical applications. Chapters provide basic introduction of TGF- signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF- signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Categories: Biology Molecular
Year :2015
Publisher : Humana Press
Language : English
N° Of Pages : xii+394
File Info : pdf 12 Mb