Methods in Molecular Biology 271B Cell Protocols [1ed.]9781588293473, 1588293475, 1592597963
Description:Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. In B Cell Protocols, highly qualified investigators who are either the inventors or expert users of their methods provide a diverse collection of protocols that range from the latest inventions and applications to classic, but still frequently used, methods in B cell biology. The protocols presented follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, B Cell Protocols offers both beginning and experienced researchers alike highly effective tools for exploring B-lymphocyte development and function in higher animals, as well as critical information on how best to design or modify an experimental approach that will prove productive in their own research.