Methods in Molecular Biology 301Ubiquitin-Proteasome Protocols [1ed.]9781588292520, 1588292525, 1592598951
Description:The targeted addition of ubiquitin to proteins is now known to regulate many cellular events, with the enzymes involved in protein ubiquitination linked to a variety of clinical problems, such as cancer, heart disease, and immune dysfunction. In Ubiquitin-Proteasome Protocols, hands-on leaders in the field, many of whom originally developed the methods they describe, detail cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome- dependent functions. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes.