Methods in Molecular BiologyPosttranslational modification of proteins [1sted.]9780896036789, 0896036782

Author : Christoph Kannicht
Description:Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the posttranslational modifications of protein, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation.
Categories: Biology Biotechnology
Year :2002
Publisher : Humana Press
Language : English
N° Of Pages : 336
File Info : pdf 3 Mb