Methods in Molecular BiologyProtein-ligand Interactions: Methods and Applications [1st Edition.]9781617375255, 161737525X

Author : G. Ulrich Nienhaus
Description:A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Categories: Biology Biotechnology
Year :2010
Publisher : Humana Press
Language : English
N° Of Pages : 580
File Info : pdf 6 Mb

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