MicroRNAs as Tools in Biopharmaceutical Production [1ed.]9789400751279, 9400751273, 9789400751286, 9400751281

Author : Lyne Joss, Lin Zhang, C Mark Smales (auth.), Niall Barron (eds.)
Description:Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
Categories: Biology Biotechnology
Year :2012
Publisher : Springer Netherlands
Language : English
N° Of Pages : 126[135]
File Info : pdf 2 Mb