Protein Phosphorylation: A Practical Approach (Practical Approach Series) 2nd ed [2ed.]9780199637287, 9780585483931, 0199637288

Author : D. G. Hardie (Editor)
Description:Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphorylation.
Categories: Biology Biotechnology
Year :1999
Publisher :
Language : English
N° Of Pages : 431[460]
File Info : pdf 22 Mb